Determination of icariin in Yinyangsu capsules by HPLC / 西安交通大学学报(医学版)

Objective To establish an HPLC method for testing the content of icariin in Yinyangsu capsules.Methods An Lichrospher C18(4.6mm?250mm,5?m) was used as the column,and column C18 as the protecting column.The mobile phase was a mixture of acetonitrile-20g/L acetum(30∶70) in a flow rate of 1.0mL/min.The detected wavelength was 270nm.Column temperature was room temperature and the injection quantity was 10?L.The sample was prepared when we added 50%(V/V) alcohol and then made ultrasonic extraction.Measurement was made after the prepared sample was filtrated on 0.45?m filter paper.Results Icariin in the sample was well separated.The linear range was 10.0-60.0mg/L.The linear equation was C=2.042?10-5A+0.554,while the correlation coefficient was 0.9993;the average recovery was 99.8% and RSD was 0.65%(n=6).Conclusion This method can be used in content measurement of Yinyangsu capsules.

Determination of Alkaloids in Zhidaipian Tablets by high performance liquid chromatogram / 西安交通大学学报(医学版)

Objective To establish a method of high performance liquid chromatogram(HPLC) for measuring matrine and oxymatrine in Zhidaipian Tablets.Methods A Lichrospher-NH2 column(4.6 mm?250 mm,5?m) was used with a mobile phase consisting of acetonitrile-ethanol-0.5 mol/L phosphoric acid(80∶10∶10),and in a flow rate of 1.0 mL/min.The detective wavelength was 212 nm.Column temperature was room temperature and the injection quantity was 20 ?L.Results The samples were well separated.The linear range was the same for the target(1.0-10.0 ?g/mL).The linear equations for matrine was C=1.201?10-4A +0.161,the correlation coefficient was 0.9992,and for oxymatrine was C=1.366?10-4A +0.221,while the correlation coefficient was 0.9996,respectively.The mean recovery rate were 99.9% and 99.4%,respectively.RSD was 1.48% and 4.33%,respectively(n=6).Conclusion The method is simple,reliable,accurate and can be used to the quality control of the Zhidaipian Tablets.

Determination of ferulic acid in Jianpibuxue tablets by HPLC / 西安交通大学学报(医学版)

Objective To establish a determination method of ferulic acid in Jianpibuxue tablets.(Methods)HPLC was used with Lichrospher C_(18)(4.6mm?250mm,5?m) as the column.The mobile phase was a mixture of acetonitrile-0.378mol/L acetum(30∶70) and in a flow rate of 0.8mL/min;The detective wavelength was 321nm;Column temperature was room temperature and the injection quantity was 10?L.(Results)Ferulic acid in samples was well separated.The linear range was 1.0-10.0mg/L(r=0.9996).The average recovery was 99.8% and RSD was 0.26 %(n=6).Conclusion This method can be used for quality control of ferulic acid in Jianpibuxue tablets.